Web12 dec. 2005 · Alpha-ketoglutarate dehydrogenase (α-KGDH) is a Krebs cycle enzyme, which catalyses the non-equilibrium reaction converting α-ketoglutarate, coenzyme A … Web26 okt. 2016 · Here we show that the α-ketoglutarate dehydrogenase (α-KGDH) complex is localized in the nucleus in human cell lines and binds to lysine acetyltransferase 2A (KAT2A, also known as GCN5) in the promoter regions of genes ... Macromolecules Find similar proteins by: (by identity cutoff) 3D Structure Experimental Data & Validation …
Did you know?
WebArticle Title: KAT2A coupled with the α-KGDH complex acts as a histone H3 succinyltransferase. doi: 10.1038/nature25003. Figure Lengend Snippet: a–c, f, Immunoblotting analyses were performed with the indicated antibodies. Representative images of triplicate experiments are shown. a, Nuclear DLST promotes histone H3 … Web3 jun. 2024 · Some study indicated that the α-KGDH complex is much greater effective than succinyl-CoA owing to the catalysis of the OGDH . In this study, most succinylated proteins were located in the chloroplast, cytoplasm, mitochondria and nucleus, accounting for 47, 23, 16 and 7% of all the identified proteins, respectively (Fig. 6 d).
Cells were collected and washed twice with ice-cold PBS, then resuspended in triton extraction buffer (TEB: PBS containing 0.5% Triton X-100 (v/v), 2 mM phenylmethylsulfonyl fluoride (PMSF), 0.02% (w/v) NaN3) at a cell density of 107 cells per ml. Cells were then lysed on ice for 10 min with gentle … Meer weergeven The antibodies used in the experiments are listed in Supplementary Table 9. DNase-free RNase A and propidium iodide were … Meer weergeven Parental U87, U251, and 293 cells were obtained from the American Type Culture Collection. Parental U87 and U251 cell lines used in the experiments were authenticated … Meer weergeven To identify KAT2A-dependent succinylation of lysines on histone H3, an in vitro KAT2A-succinylated sample of purified histone H3 was exhaustively acetylated … Meer weergeven PCR-amplified human OGDH, DLST, and DLD were cloned into pcDNA6V5HisB and pcDNA3.1/hygro(+)-Flag vectors. A full-length KAT2A … Meer weergeven WebMolecular Mechanism of Histone Acetyltransferase GCN5 in the Regulation of Metabolic Diseases: CHEN Hui, ZHANG You-Zhi, OUYANG Chang-Han *: School of Pharmacy, Hubei University of Science and Technology, Hubei Key Laboratory of Cardiovascular, Cerebrovascular and Metabolic Disorders, Xianning 437100, Hubei, China
Web29 mrt. 2016 · KGDH complex activity in SH SY5Y cells was assayed following 15-min exposures of the cells to dopamine, chlorodopamine, or HOCl . Exposure to 200 μM dopamine for 15 min had no appreciable effect on KGDH complex activities in SH SY5Y cells consistent with its effect on cell survival ( Figure 5 ). Web12 apr. 2024 · Complex I activity. Complex I activity in whole cells was measured using the Complex I Enzyme Activity Dipstick Assay Kit (ab109720, ABCAM, Cambridge, MA, USA) according to manufacturer’s instructions. Three biological replicates were used per measurement. Results are expressed as enzyme activity with respect to control.
WebHere, we examined the hydrogen peroxide (H2O2) producing capacities of pyruvate dehydrogenase (PDH), α-ketoglutarate dehydrogenase (KGDH), proline dehydrogenase (PRODH), glycerol-3-phosphate dehydrogenase (G3PDH), succinate dehydrogenase (SDH; complex II), and branched-chain keto acid dehydrogenase (BCKDH), in cardiac and liver …
Web24 okt. 2006 · KGDH and PDH complex activities in patient mitochondria also were decreased but less severely (Fig. 7 B and D). The lactate level in the medium was measured in both MEFs and lymphoblasts. In mutant MEFs, a 6.3× increase and in patient lymphoblasts, a 1.6× increase in lactate was seen (Fig. 13, which is published as … brightlingsea holiday parkWebα-Ketoglutarate Dehydrogenase (α-KGDH) (EC 1.2.4.2) is a key enzyme in the citric acid cycle. It forms an enzyme complex with dihydrolipoamide succinyl transferase (E2) and dihydrolipoamide dehydrogenase (E3). α-KGDH converts α-ketoglutarate into succinyl-CoA in the presence of NAD and CoA. brightlingsea indianWebThe KGDC complex mainly resides in mitochondria, but one of its components, alpha ketoglutarate dehydrogenase (KGDH), possesses a putative nuclear localization signal … brightlingsea hotelsWebPDH, a-KGDH and LAD in soni cated fibroblasts cultured from Fried reich's patients: at variance with the data of Rodriguez-Budelli et al., (1978) the kinetics of LAD was comparable in the controls' and the patients' cells. Moreover, Stumpf and Parks (1979) showed that oxidation rates by whole cell suspensions of many substrates, can you fry marinated chickenWeb5 jan. 2024 · Although succinyl-CoA can be converted from α-KG by the α-KGDH complex, which is composed of oxoglutarate dehydrogenase (OGDH), dihydrolipoyl succinyltransferase (DLST), and dihydrolipoyl dehydrogenase (DLD), in the mitochondria, α-KGDH is also distributed in the nucleus in an NLS of DLST-dependent manner in … brightlingsea holiday cottagesWebDecreased α-KGDH complex activity has been associated with oxidative stress and neurodegenerative disorders such as Alzheimer′s and Parkinson′s diseases. Application. α-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit has been used to measure the enzyme activity of α-ketoglutarate dehydrogenase in samples. brightlingsea infant schoolWeb13 nov. 2007 · The 2-oxoglutarate dehydrogenase complex is composed of OGDH (2-oxoglutarate dehydrogenase; E1), DLST (dihydrolipoamide succinyltransferase; E2) and … can you fry mash potato