WebIntramolecular fluorescent arsenical hairpin (FlAsH) BRET, wherein a Renilla luciferase (Rluc) donor excites a fluorescent arsenical acceptor placed within the protein by mutational insertion of a tetracysteine tag motif, can be used to monitor stimulus-induced changes in protein conformation.
NanoBRET™ Nano-Glo® Detection Systems - Promega
WebMultiple GPCR functional assays have been developed based on Förster resonance energy transfer (FRET) and bioluminescence resonance energy transfer (BRET) sensors to monitor the conformational... WebJan 10, 2024 · The BRET assay, based on the interaction with Calmodulin, was successfully extended to TRPV3 and TRPV4 channels. ... (FlAsH) (45). Adapting the FlAsH/BRET approach to other channels would require extensive studies to determine how to insert the FlAsH sequence into the channel structure to yield optimal variation in the … bird in the bush
Probing Arrestin Function Using Intramolecular FlAsH-BRET
WebFeb 26, 2024 · BRET imaging techniques are broadly used for analysis of protein interactions and several other methods for illustration of bacterial cultures, plant tissues, and mammalian cell suspensions. An... WebMar 26, 2024 · BRET assays have been used to observe an array of biological functions including ligand binding, intracellular signaling, receptor-receptor proximity, and receptor trafficking, however, BRET assays can theoretically be used to monitor the proximity of any protein or molecule for which appropriate fusion constructs and/or fluorophore conjugates ... WebApr 13, 2024 · Whereas the FlAsH BRET method indicated that IP15 induced closer contacts between the N terminus of ADGRG2 and the ECL1 and ECL2 regions, binding of the antagonist IP15-F601D led to the... bird in the bottle